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Lonza 96孔模塊優(yōu)勢(shì)
1、高效轉(zhuǎn)染——采用上市20年的Lonza（原Amaxa）專利Nucleofector細(xì)胞核轉(zhuǎn)染技術(shù)，結(jié)合優(yōu)化的電轉(zhuǎn)染程序、細(xì)胞特異性轉(zhuǎn)染試劑、無(wú)鋁離子毒害的新型聚合物電極，同時(shí)提高細(xì)胞轉(zhuǎn)染效率和轉(zhuǎn)染后細(xì)胞活率。
2、快速優(yōu)化——真正的96個(gè)孔獨(dú)立轉(zhuǎn)染，可用于不同細(xì)胞轉(zhuǎn)染或同一細(xì)胞不同轉(zhuǎn)染條件的摸索，通過(guò)較低的細(xì)胞、轉(zhuǎn)染試劑、轉(zhuǎn)染耗材成本，短時(shí)間快速推進(jìn)實(shí)驗(yàn)進(jìn)程。
3、兼容性——96孔模塊摸索的轉(zhuǎn)染條件，可方便地應(yīng)用于4D核轉(zhuǎn)儀的X模塊和LV大體積轉(zhuǎn)染模塊、或Lonza 384孔高通量轉(zhuǎn)染系統(tǒng)，簡(jiǎn)單實(shí)現(xiàn)轉(zhuǎn)染規(guī)模的線性放大。
4、自動(dòng)化處理——96孔模塊可集成于液體處理工作站中，實(shí)現(xiàn)自動(dòng)化處理，提升實(shí)驗(yàn)效率，減少人工誤差。
參考文獻(xiàn)
1. Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Thomas Gaj, Brett Staahl, Gonçalo Rodrigues, et al. Nucleic Acids Research, Volume 45, Issue 11, 20 June 2017, Page e98
2. Nucleofection-based gene targeting in human pre-B cells. Aya Kurosawa, Shinta Saito, Mikako Mori, Noritaka Adachi. Gene, Volume 492, Issue 1, 15 January 2012, Pages 305-308
3. Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation. Robert Bowles, Sonali Patil, Hanna Pincas, Stuart C. Sealfon. Immunology and Infection, July 8, 2011, doi: 10.3791/2766
4. An Engineered Zinc Finger Protein Activator of the Endogenous Glial Cell Line-Derived Neurotrophic Factor Gene Provides Functional Neuroprotection in a Rat Model of Parkinson's Disease. Josee Laganiere, Adrian Kells, Jeffrey Lai, et al. Neuroscience, 8 December 2010, 30 (49) 16469-16474
5. An RNA Interference Screen Reveals a Critical Role for Mcl-1 In Survival of CML Progenitor Cells. Su Chu and Ravi Bhatia. Blood, Volume 116, Issue 21, 19 November 2010, Page 1203
更多Lonza 4D-Nucleofector 96孔轉(zhuǎn)染模塊信息，歡迎點(diǎn)擊右側(cè)在線咨詢
——Lonza中國(guó)一級(jí)代理商，北京澤平
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